Bone marrow derived cells decrease inflammation but not oxidative stress in an experimental model of acute myocardial infarction

Abstract

Aims Bone marrow cell (BMC) therapy is thought to exert beneficial effects on the infarcted heart. We assessed cardiac function and its correlation with redox status and inflammation in cardiac tissue early post-AMI in rats treated with BMC. Main methods Male Wistar rats (8-week-old) were randomized into four groups: Sham-operated (S); AMI; S + treatment (ST) and AMI + treatment (AMIT). Therapy with BMC was carried out immediately post-experimental left anterior coronary artery ligation induced-AMI, and assessments made 48 h later. Cardiac function and morphometrics were evaluated by echocardiographyc parameters in vivo. Cardiac tissue tumor necrosis factor (TNF)-α and interleukin (IL)-6 were measured by Western Blot. Oxidative stress parameters including reduced (GSH) and oxidized (GSSG) glutathione ratio, hydrogen peroxide level, lipid and protein oxidation, superoxide dismutase, catalase and glutathione peroxidase activities were measured spectrophotometrically. Key findings Ejection fraction was lower in infarcted groups and did not improve in BMC-treated animals: AMI (51 ± 5%) vs. S (74 ± 7%) and AMIT (56 ± 10%) vs. ST groups (73 ± 3%). Both TNF-α and IL-6 myocardial expression increased post-AMI and were reduced following BMC therapy. Nonetheless, there was a decrease in GSH/GSSG ratio in infarcted groups which was greater in BMC-treated groups: AMI (8.21 ± 3.8) vs. S (14.61 ± 3.4) and AMIT (2.1 ± 0.7) vs. ST (4.7 ± 1.5). Significance The data suggest that BMC promoted a redox status favorable to the oxidation of the pro-inflammatory cytokines in the myocardium, exerting an anti-inflammatory-like effect.