Abstract
The purpose of this study was the comparison between capacity of bone marrow cells on the one hand, and of mature lymphocytes and monocytes on the other hand, to induce immunotolerance by inoculation in COBB 500 hybrids at different embryonic stages. Antigeni c material was obtained by aspiration of tibial and femoral bone marrow, respectively by blood sampling from the donor birds (Ross hybrids).We used 240 embryonated eggs: 60 eggs for in ovo inoculation with bone marrow cells suspension in t he 5 th day of embryo development and 60 for inoculation in the same way and day with mature lymphocytes and monocytes suspension; the rest of biol ogic material was inoculated with these suspensions in a llantoidal vessel in the 8 th day of the embryo. Seven days after the full-thickness skin grafts transplantation (fro m the donor birds) the lymphocyte T subsets of the recipient birds were determined by flow cytometry and immunomagnetic sorting. Also, all the skin grafts were periodica lly monitored for macroscopic characteristics. This exp eriment demonstrates the superiority of bone marrow cells in immunotolerance induction in birds after inoculatio n in 5 th day of embryonic development.
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