Abstract
Both a C 1 and a C 18 saturated hydrocarbon have been bonded to 75-μm capillaries for high-voltage capillary electrophoresis separations. The performance of these bonded phases has been compared with unbonded capillaries under a variety of experimental conditions. The bonded phases were prepared by a flow-through procedure at room temperature, and the reproducibilities of the electroosmotic flow for the C 1 and C 18 capillaries were 6 and 3%, respectively. Both of the bonded phases reduced interactions between the silica surface and positively charged ions, but for larger hydrophobic ions sorption and peak tailing were observed on the C 18 phase. In the presence of sodium dodecylsulfate and sodium decanesulfonate it was possible to control the electroosmotic flow over a wide range. The separation of lanthanide metal ions is illustrated, and improved resolution and reduced surface interactions are shown for the bonded phases.
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