Abstract
Abstract High pressure liquid chromatographic methods were developed to separate 20-hydroxyecdysone and juvenile hormone I in the pupal and adult stages of the boll weevil. Minimum detectable levels were 4 ng for 20-hydroxyecdysone and 4 ng for juvenile hormone. No juvenile hormones were detected in male boll weevils, whereas titers were readily determined in female boll weevils. The ecdysteroid, 20-hydroxyecdysone was present in pupae (not sexed), in adult males from 3 to 6 days of age, and in adult females from days 2 through 6.
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