Abstract
Flux through glycolysis and the pentose shunt pathway and the rate of glucose oxidation to CO₂ were examined as functions of body mass in the sea anemone Metridium senile. Minced tissues from anemones acclimated to 5°C and 15°C were incubated in media containing isotopically labeled glucose compounds to determine pathway fluxes. Double-logarithmic regressions showed that the specific rate of glucose oxidation declined significantly with increasing anemone mass at 15°C (the mass exponent in the expression $Y = aM^{b}$ is b, and b-1 =-.210; P = .0321) but was mass-independent at 5°C. Pentose shuntflux scaled negatively with anemone mass at 5°C (b-1 =-. 063; P = .0124) but was mass-independent at 15°C. Regression of glycolytic flux versus anemone mass was significant at 5°C (b-1 =-.052; P = .0218) but not at 15°C, which was opposite the pattern observed for the glucose oxidation rate at these temperatures. Therefore, glycolytic flux and the glucose oxidation rate scaled independently. The mass exponents for ...
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