Abstract
Abstract Small variations in incubation temperature can significantly affect the final BOD result. Thirteen incubators were examined by their operators and found to display wide variations in temperature. Only three were able to conform to the standard incubation temperature and limits of 20° ± 0.5 deg.C. Temperature variation in the incubators tested varied from 0.3 to 3.0 deg.C. Temperature uniformity within a single incubator was studied using a matrix of thermocouples. While the temperature within individual BOD bottles remained reasonably constant over a 24 h period (0.2–0.6 deg.C), the mean bottle temperature varied from 18.7 to 20.9°C depending on the position within the incubator. Other factors such as stocking density and opening the incubator door are also considered. Recommendations are given to ensure minimum deviation from the recommended incubation temperature of 20° ± 0.5 deg.C.
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