Abstract
Burn injuries are one of the key medical and social problems. Despite the significant achievements in combustiology, the slow healing and the appearance of infection are the key problems in burn patients, which lead to a longer hospitalization period, to reduction of life quality and to emotional disorders. Up to 70% of all complications after thermal trauma (TT) are connected with infection – first of all, pneumonia, infections of urinal tract. The forming of infectious complications, including sepsis, after TT is associated with excessive immunosuppressive reactions, as compensation for a long, stable proinflammatory response, in particular, owing to hyperproduction and effects of IL-10, IL-4, TGF-β. Aim: to study the influence of systemic and local usage of MT with original dermal film (DF) on reparation and serum cytokine concentration indicators in dynamics of experimental TT. The study was conducted using 84 rats – males of Wistar line, which were divided randomly into 4 groups: 1st group (n = 12) – intact monitoring, 2nd group (n = 30) – animals with TT, 3rd group (n = 21) – animals with TT and DF with MT use on the region of burn, 4th group (n = 21) – animals with TT and intraperitoneal injection of MT. To model TT of IIIA degree and relative area 3,5%, isolated skin area of interscapular area was immersed in distilled water at a temperature of 98-99 °С at 12 s. The DF with MT (at a concentration of 0.005 g/g) on 12 sm2 – area in 3rd group was used daily for 5 days. The MT was injected intraperitoneally daily at the dose of 10 mg/kg for 5 days. The wound area was calculated, the interleukin-4 (IL-4), tumor necrosis factor alpha (TNFα), interferon-gamma (IFNγ) were determined in serum on 5th,10th and 20th day from the moment of TT induction in each group. During experimental TT in dynamic monitoring from 5th to 20th day the absolute and relative areas of wound defect are reduced, because of that the epithelization speed and its part of area reduction are progressively increasing, on 5th,10th and 20th day the concentration of TNFα and IL-4 in serum is increasing with maximum values on 10th day of monitoring. Local usage of MT in DF during TT accelerates the healing of burn wound and lowers the TNFα and IL-4 concentration in serum on 5th, 10th and 20th day. Intraperitoneal use of MT during TT accelerates the healing of burn wound and lowers the TNFα and IL-4 concentration in serum on 5th and 20th day. The reparation accelerating effect of MT during TT is more expressed in locale usage in DF rather than using intraperitoneal injection.
Highlights
Burn injuries are one of the key medical and social problems
Development of infectious complications, including sepsis, after thermal trauma (TT) is associated with excessive immunosuppressive reactions to compensate for long-lasting stable proinflammatory response owing to overproducted IL-10, IL-4, TGF-β [2]
In dynamics of TT, the concentration of TNFα and IL-4 in serum on day 10 vs day 5 was higher (р < 0.05) and didn’t differ from those on day 20, that allows to note about peak level of TNFα and IL-4 on day 10 of monitoring
Summary
Burn injuries are one of the key medical and social problems. Despite the significant achievements in combustiology, slow healing and appearance of infection are the key issues in burn patients, which lead to prolonged hospitalization period, lowered quality of life and emotional disorders [1]. Мелатонин при термической травме Melatonin in thermal trauma of safe necrectomy and wound closures [3]. In this regard, endogenic homeostasis regulators, which can interfere in mounting immune response to TT and to be involved as immunocorrectors attract special interest. We showed that erythropoietin app lied locally and systemically as well as local EGF administration in TT accelerate healing of burn injury and restoration of the immune status in clinical and experimental conditions [4, 5]. Skin cells produce MT, so that its metabolites are found in keratinocytes, melanocytes, dermal fibroblasts [7]. MT1, MT2, RORα receptors were found in keratinocytes, skin fibroblasts, the cells of the hair follicle and dermal blood vessels, and melanocytes [8]. Aim: to study an influence of systemic and local MT application with original dermal film (DF) on repair and dynamic serum cytokine profile in experimental TT
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