Abstract
Oxidative stress (OS) escalation associated with thermal trauma (TT) and pleiotropic effects of melatonin (MT) suggest a study of protective properties of the latter when applied as part of a novel dermal film (DF) to skin burns. This work aimed to assess the content of OS markers in the skin subjected to experimental TT and treated with DF with MT. Third A degree TT (area of 3.5%) were modeled by immersing a patch of skin in boiling water. Twelve cm2 of DF with 5 mg/g of MT were applied daily for 5 days. The parameters calculated were wound's area and epithelializatiohon rate. The products monitored in the burn wound were lipid peroxidation (LPO) products in heptane and isopropanol phases of the lipid extract and protein oxidative modification (POM) products, the modification being spontaneous and metal-dependent. With TT in the wound, the content of secondary and end LPO products in heptane and isopropanol phases increased on the 5th and 10th days; the total content of POM products grew on the 5th day (primary products, neutral) and on the 10th day (primary and secondary products, neutral). Application of DF to a TT wound reduced the burn area, increased the epithelialization rate (by the 10th day, the median went from 1.90% to 6.57%; p < 0.05), reduced the content of secondary and end LPO products in isopropanol phase (by the 10th day, the median went from 0.007 to 0.004 u.o.i; p < 0.05), reduced the total content of OMP products, namely that of primary neutral products — on the 5th day, of primary and secondary neutral products — on the 10th day. With TT present in the context of MT application, the burn area showed presence of secondary LPO products in heptane and isopropanol phases, LPO end products in isopropanol phase, POM products in the wound (basic and neutral primary/secondary POM products).
Highlights
There are about 250,000 burn cases registered in the Russian Federation annually
On the 5th and 10th days, the content of lipid peroxidation (LPO) products registered in the wound skin homogenate changed (Fig. 1, 2)
It is predominantly reserve lipids that accumulate in the heptane phase, while those accumulating in the isopropanol phase are membrane phospholipids
Summary
There are about 250,000 burn cases registered in the Russian Federation annually. About 80% of them are associated with thermal trauma (TT) [1]. Adverse outcome and development of TT-related complications depend on the injury's area and depth, severity of inflammation, acute phase response and immune reactions, and on the local redox status [3]. Identification of aldehyde- or ketonecontaining carbonyl derivatives of proteins (POM products) and LPO products (as markers of oxidative stress and performance of antioxidants) is of great interest [6]. There is a number of types of wound dressings applied as treatment of localized thermal skin injuries. Such dressings ease pain during bandaging, make the wound moist and promote cell differentiation and effective intercellular interaction, and shorten treatment time.
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