Abstract
PurposeThe long non-coding RNA MALAT1 is a predictive marker in several solid tumors with highly conserved sequences. However, the role of non-coding RNA in development of laryngeal or hypopharyngeal cancer remains unclear.MethodsTumor tissues and adjacent non-cancer tissues of 24 patients were collected. We detected the expression of MALAT1 in laryngeal cancer tissues and hypopharyngeal cancer tissues. Moreover, we developed a MALAT1 silencing model in human laryngeal tumor cells by transfecting MALAT1 small interfering RNA into human laryngeal carcinoma cell line Hep-2 and pharyngeal carcinoma cell line FaDu with Lipofectamine 2000 system. Cell cycle analysis, Cell Counting Kit-8 assay, Transwell assay, quantitative reverse transcription PCR, and wound-healing assays were performed to evaluate the impact of MALAT1 depletion on laryngeal or hypopharyngeal cancer cell’s growth, proliferation, apoptosis, invasion and migration.ResultsMALAT1 was significantly up-regulated in laryngeal and hypopharyngeal carcinoma cells. MALAT1 down-regulation induced the increased apoptosis of both cell lines and suppressed cells’ proliferation. Cells were arrested in G1/G2 phase and cells of S phase were significantly decreased. Down-regulation of MALAT1 expression can also inhibit the migration and invasion of laryngeal squamous cell carcinoma cell (Hep-2) and hypopharyngeal cancer cell (FaDu).ConclusionIn summary, our deactivation model of MALAT1 disentangled the active function of it as a regulator of gene expression governing the hallmarks of laryngeal and hypopharyngeal cancer. Blocking this long non-coding RNA may restrain the development of laryngeal cancer.
Highlights
Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignancies of head and neck, accounting for 7.9–35% of the otolaryngology tumors [1]
MALAT1 has been investigated in multiple human cancers [5,6,7], it is rarely known whether it is associated with laryngeal cancer development in some mechanisms
The ISH data illustrated that MALAT1 expression was significantly increased in laryngeal cancer and hypopharyngeal tissues as compared with that in the normal tissues (P < 0.05, Fig. 1)
Summary
Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignancies of head and neck, accounting for 7.9–35% of the otolaryngology tumors [1]. The long non-coding RNAs (lncRNAs) are defined by length, ranging from 200 nt to 138 kb. Like messenger RNAs, they are transcribed by RNA polymerase II and can be modified by diverse ways. LncRNAs lack a significant open reading frame, which can be transcribed from the sense or antisense orientation [2]. LncRNAs are important molecular elements in eukaryotic cells, and play key roles in various aspects of mRNA stability, transcriptional regulation, protein transport, RNA processing and modification. Piling up evidence indicates that lncRNAs play a critical role in multiple cancers development and progression. Lung adenocarcinoma metastasis-associated transcript 1 (MALAT1) is one of the earliest discovered
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