Abstract
Abstract Blast cell colonies (BCC) were initially identified on day 16 of culture of bone marrow cells of normal mice (Nakahata and Ogawa, 1982a). The growth of colonies was supported by the medium conditioned by pokeweed mitogen-stimulated spleen cells (PWM-SCM). The BCC were identified as a small diffuse collection of round refractile cells without a central core. Upon staining, the cells in the BCC revealed no signs of cytoplasmic differentiation and the nuclei of the cells were immature, showing open chromatin and often one or more nucleoli. When BCC were harvested individually and plated in secondary cultures, the cells in the BCC revealed very high replating efficiencies, sometimes nearing 100%. In the secondary culture, a large number of multilineage colonies were seen.
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