Abstract
<i>bla</i><sub>NDM-1</sub>–positive<i>Klebsiella pneumoniae</i>from Environment, Vietnam
Highlights
Editor: The bla NDM-1 gene, which produces the New Delhi metallo-β-lactamase (NDM-1) enzyme, confers resistance to the carbapenem class of antimicrobial drugs and can be transferred among different types of bacteria
To isolate and identify the phenotype and genotype of bla –positive NDM-1 bacteria, we repeatedly spread the 20 culture swab specimens onto Muller-Hinton agar (Nissui, Tokyo, Japan) containing 100 mg/L vancomycin (Nakalai, Kyoto, Japan) plus 0.5 mg/L
All 6 K. pneumoniae isolates were positive for blaTEM and blaCTX-M variants by PCR; these variants were confirmed as blaTEM-1 and blaCTX-M-3 by sequencing
Summary
Editor: The bla NDM-1 gene, which produces the New Delhi metallo-β-lactamase (NDM-1) enzyme, confers resistance to the carbapenem class of antimicrobial drugs and can be transferred among different types of bacteria. During September 2011, we collected paired swab samples (1 for PCR, 1 for culture) of seepage water from 20 sites (rivers, lakes, and water pools in streets) within a 10-km radius of central Hanoi, Vietnam. Two NDM-1 samples were positive for bla ; NDM-1 these 2 samples were collected from the same river (Kim Nguu River) but at sites 3 km apart.
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