Black Seed Thymoquinone Improved Insulin Secretion, Hepatic Glycogen Storage, and Oxidative Stress in Streptozotocin-Induced Diabetic Male Wistar Rats.

Heba M A Abdelrazek,Aaser M Abdelazim,Hend M Tag,Muhammad A A Muhammad,Omnia E Kilany

doi:10.1155/2018/8104165

Heba M A Abdelrazek, Aaser M Abdelazim + Show 3 more

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https://doi.org/10.1155/2018/8104165

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Abstract

Diabetes mellitus is one of the metabolic diseases having several complications. Nigella sativa oil (NSO) might have beneficial effects in the treatment of diabetic complications. Thirty-two mature male Wistar rats were equally divided into four experimental groups: control, control NSO 2 mL/kg, streptozotocin- (STZ-) induced diabetic, and diabetic (STZ-induced) treated with oral NSO 2 mg/kg for 30 days. Fasting blood glucose (FBG), insulin, and lipid profile levels were determined. Pancreatic and hepatic tissues were used for catalase and GSH. Histopathology, hepatic glycogen contents, insulin immunohistochemistry, and pancreatic islet morphometry were performed. NSO 2 mL/kg was noticed to decrease (P < 0.05) FBG and increase (P < 0.05) insulin levels in diabetic rats than in diabetic nontreated animals. Lipid profile showed significant (P < 0.5) improvement in diabetic rats that received NSO 2 mL/kg than in the diabetic group. Both pancreatic and hepatic catalase and GSH activities revealed a significant (P < 0.05) increment in the diabetic group treated with NSO than in the diabetic animals. NSO improved the histopathological picture and hepatic glycogen contents of the diabetic group as well as increased (P < 0.05) insulin immunoreactive parts % and mean pancreatic islet diameter. NSO exerts ameliorative and therapeutic effects on the STZ-induced diabetic male Wistar rats.

Highlights

Diabetes mellitus (DM) is considered as one of the most common chronic metabolic diseases characterized by increased blood glucose due to insulin resistance, insulin deficiency, or both [1]
Hyperglycemia and lipid profile abnormalities are the main clues for diagnosis of DM metabolic disorders [2]
The body weights of the STZ-induced diabetic group rats reduced significantly (P < 0 05) than did those of the control nondiabetic rats at the 3rd and 4th weeks of the experiment

Summary

Introduction

Diabetes mellitus (DM) is considered as one of the most common chronic metabolic diseases characterized by increased blood glucose due to insulin resistance, insulin deficiency, or both [1]. DM is associated with vascular, metabolic, neuropathic, and nephropathic disorders. Hyperglycemia and lipid profile abnormalities are the main clues for diagnosis of DM metabolic disorders [2]. Hyperglycemia is a consequence of the inability of the cells to utilize glucose and/or skeletal muscles and liver are not capable of glycogen storage [3]. The persistent hyperglycemia in DM promoted oxidative stress through the formation or release of reactive oxygen species (ROS) and depletion of antioxidant reserve. Oxidative stress is the main cause of cardiovascular disease that results in mortalities [4, 5]

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