Abstract
Abstract The biotransformation of isoimperatorin ( 1 ) by Cunninghamella blakesleana AS 3.970 yielded 6 novel products, 14-hydroxyl-isoimperatorin ( 2 ), 11-carbonyl-14-hydroxyl isoimperatorin ( 3 ), 11-carbonyl-14-hydroxyl-12,13-dihydrogen-iso-imperatorin ( 4 ), 14-hydroxyl-12,13-dihydrogenisoimperatorin ( 5 ), isoimperatorin-14-O- β - d -mannoside ( 6 ) and isoimperatorin-14-O- β - d -glucoside ( 7 ), respectively. The chemical structures of these metabolites were elucidated based on extensive spectral data including 2D NMR and HRMS. The hydroxylation, hydrogenation, carbonylation and glycosylation reactions of 1 by C. blakesleana AS 3.970 were observed in the present study. In addition, anti-osteoporosis activities of substrate and all transformed products were evaluated by using MC3T3-E1 cells. Our results suggested that hydroxylation or glycosylation of C-14 would enhance anti-osteoporosis activity significantly.
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