Biosynthesis of Zinc Oxide-Zerumbone (ZnO-Zer) Nanoflakes Towards Evaluating Its Antibacterial and Reactive Oxygen Species (ROS)-Dependent Cytotoxic Activity.

Abstract

Being the second most prevalent metal oxide, zinc oxide (ZnO) nanomaterials have been widely studied and found to exhibit promising applications in various domains of biomedicine and agriculture. Considering the enhanced bioactivities displayed by secondary metabolite (SM) derived ZnO nanomaterials, present study was undertaken to evaluate the efficacy of ZnO nanoflake (NF) derived from Zerumbone (Zer), a sesquiterpenoid from Zingiber zerumbet rhizome with diverse pharmacological properties. ZnO NF prepared by homogeneous precipitation method using ZnSO4.7H2O (0.1M) and NaOH (0.2M) as precursors with and without the addition of Zer (0.38 mM) were characterized by powder UV-visible spectroscopy, X-ray diffraction (XRD), FT-IR spectroscopy and Field emission scanning electron microscope (FESEM) analysis. Optical and physical properties of ZnO-Zer NF were found to match with the typical ZnO nanomaterial properties. XRD analysis revealed reduction in size (15nm) of the green synthesized ZnO-Zer NF compared to ZnO NF (21nm). ZnO-Zer NF displayed linear correlation between concentration and antimicrobial activity to Salmonella typhi, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Determination of cytotoxic potential of the synthesized ZnO-Zer NF in cervical cancer cells (HeLa) showed higher cytotoxicity of ZnO-Zer NF (39.32 ± 3.01%) compared to Zer alone (27.02 ± 1.22%). Present study revealing improvement in bioactivity of Zer following conjugation with ZnO NF signifies potential of NF formation in improving therapeutic application of Zer that otherwise displays low solubility limiting its bioavailability.