Abstract

-desaturase gene. PCR showed the presence of the transgenes within the genome of selected plants, with RT-PCR confirming gene expression. Gas Chromatography of fatty acid methyl esters extracted from freeze-dried leaves of transgenic plants quantified the synthesis of omega-6 arachidonic acid and its precursors eicosadienoic and dihomo-γ-linolenic acids, and omega-3 eicosapentaenoic acid together with its precursors eicosatrienoic and eicosatetraenoic acids. This is the first report of the production of VLCPUFAs in a leafy vegetable. Since VLCPUFAs are the precursors of prostaglandins, the formation of prostaglandins was also investigated in chicory following a second transformation event using the PGHS-1 gene from

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