Abstract
Shikimic acid-G-C 14 (i.e. generally labelled), anthranilic acid-G-C 14, indole-2-C 14, serine-3-C 14 and indolyllactic acid-β-C 14 were found to be a good precursor of bound tryptophan in young barley shoots. It is likely that tryptophan is formed in barley by the same route established for Escherichia coli. 1–5 Tryptophan was readily converted to gramine in agreement with the prior observations of Marion et al. 10–13 and compounds converted readily to tryptophan were fairly good precursorsof gramine. Serine-3-C 14 gave gramine with about twice as much C 14 in the methyl groups as in the rst of the molecule, whereas gramine formed from indole-2-C 14 or anthranilic acid-G-C 14 was not labelled in the methyl groups. Indolylacetic acid, indolylacetonitrile, indolylacetaldehyde, indolylacrylic acid and tryptamine were not readily converted to either tryptophan or gramine. Gramine was not converted back to tryptophan. None of the compounds tested appeared to be intermediates in the formation of gramine from tryptophan.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call