Abstract
Rat hepatocyte suspensions were separated into parenchymal and nonparenchymal (endothelial-rich) cells and the conversion of arachidonate into prostaglandin E was studied. Most of the intracellular labeled arachidonate was incorporated into phospholipids. Fractionation of parenchymal cell phospholipids by thin-layer chromatography showed the highest specific activity in the phosphatidylserine plus phosphatidylinositol zone followed by phosphatidylcholine and phosphatidylethanolamine; sphingomyelin was not labeled. The conversion of arachidonate into prostaglandins was 1% and 4.3% in parenchymal and nonparenchymal cells respectively. Nonparenchymal cells synthesized 5-fold more prostaglandin E-like material than parenchymal cells. Quantitation of prostaglandin E biosynthesis by radioimmunoassay revealed that in the presence of cold arachidonate sinusoidal cells synthesized 1200 pg of immunogenic prostaglandin E per mg protein, in parenchymal cells the figure was 175 pg per mg protein. Parenchymal cells had little effect on platelet aggregation, whereas nonparenchymal cells inhibited aggregation in a dose-dependent fashion. This suggests that sinusoidal liver cells synthesize a prostacyclin-like compound in addition to prostaglandin E 2.
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