Abstract
Conditions for the cultivation of Rhizopus oligosporus have been investigated. It was found that addition of 25% of soybean paste to the potato medium improved growth. Riboflavin production was higher in the soybean-potato (soy-potato) medium than in the potato medium in proportion to the increase in the weight of the fungus. Biotin production per unit weight of the fungus was higher in the soy-potato than in the potato medium. The thiamine, riboflavin, and biotin content of the fungus increased during fermentation in proportion to weight or nitrogen content. Concentration of riboflavin and biotin in the medium also increased. The thiamine content of the medium decreased indicating that it was taken up from the medium by the fungus rather than synthesized.
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