Abstract
Biosynthesis of (R)-2,3-di-O-phytanylglycerol (1), the core lipid of the membranes in the strict anaerobe Methanobacterium thermoautotrophicum, was studied in cell-free preparation. Two prenyltransferase activities were separated by ultracentrifugation. The cytosolic fraction contained activity for (S)-3-O-geranylgeranylglyceryl phosphate synthase (CGGP synthase), which catalyzes alkylation of (S)-glyceryl phosphate [(S)-3-OP] by geranylgeranyl diphosphate (5-OPP), while the pellet contained activity for (S)-2,3-O-digeranylgeranylglyceryl phosphate synthase (DGGGP synthase), which catalyzes alkylation of (S)-3-O-geranylgeranylglyceryl phosphate [(S)-12-OP] by 5-OPP
Published Version
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