Abstract

A facile ecofriendly biological approach for the synthesis of selenium nanoparticles using the probiotic Lactobacillus acidophilus bacteria and its characterization is reported in this paper. The synthesized selenium nanoparticles were characterized using UV spectrometer, X-ray diffraction and transmission electron microscopy. The results of X-ray diffraction and transmission electron microscopic studies showed that the nanoparticles were in the uniform size range of 15-50 nm. The present study showed that no chemical changes occurred in selenium nanoparticles during the wet sterilization process and therefore, the wet sterilization method can effectively use to recover the elemental selenium from bacterial cells.

Highlights

  • Bio nanotechnology is an emerging important technical tool for the development of reliable ecofriendly methodology for the synthesis of materials in nanoscale using biological sources

  • While chemical approaches are the most popular methods for the production of nanoparticles some chemical synthesis protocols involve the use of toxic chemicals which are hazardous to both the environment and the biological system in which they are used

  • We report a facile, economical and green protocol to synthesize Se nanoparticles (SeNPs) using Lactobacillus acidophilus bacteria using wet sterilization process, which, holds promising alternative for the large-scale commercial synthesis of selenium nanoparticles

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Summary

Introduction

Bio nanotechnology is an emerging important technical tool for the development of reliable ecofriendly methodology for the synthesis of materials in nanoscale using biological sources. Nano sized particles possess unique properties due to their larger surface to volume ratio and higher surface energy. Metal nanoparticles have diverse broad ranging tremendous applications in the field of chemistry, electronics, diagnostics, biomedical and material science. Production of nanomaterials can be achieved through conventional physical and chemical methods. Physical methods employ cumbersome high cost equipment and techniques involving elevated temperatures and high pressures. While chemical approaches are the most popular methods for the production of nanoparticles some chemical synthesis protocols involve the use of toxic chemicals which are hazardous to both the environment and the biological system in which they are used

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