Biosynthesis and Characterization of Silver Nanoparticles-Synthesized using Extracts of Mangrove Sonneratia caseolaris leaves

Abstract

The bottom-up method offers the optimum ways to synthesize nanoparticles efficiently and in an environmental friendly way. The phenolic compound contained in plant extract can be used to synthesize nanoparticles. The purpose of this study was to determine the ability of Sonneratia caseolaris mangrove leaf extract to synthesize silver nanoparticles (AgNPs). Further characterization and investigation of the antibacterial activity were also scrutinized. The first stage was the extraction of mangrove Sonneratia caseolaris leaves. The second stage was biosynthesis of the silver nanoparticles using three (1, 5 and 10 mM) concentrations of AgNO3. The third stage was investigating its characteristics and its antibacterial activity. The results showed that mangrove Sonneratia caseolaris leaf extract could produce biosynthesis nanoparticles from AgNO3. The best reaction for silver nanoparticles was 10 min reaction times with a concentration of 5 mM AgNO3. In the characterization with FTIR, the peak results obtained from biosynthesis were 3431.89, 1632.43, 1508.99, 1429.92, 928.46, 706.66 and 579.37. The size of silver nanoparticles ranged from 90-200 nm. The antibacterial activity of AgNPs dominantly inhibited gram-negative bacteria Escherichia coli and Salmonella thyphimurium.