Abstract
Mono- and bienzyme-based amperometric biosensors were used for the direct enantioanalysis of l- and d-pipecolic acid in serum samples. The biosensors were designed using physical immobilization of l-amino acid oxidase and/or horseradish peroxidase for the assay of l-pipecolic acid, and d-amino acid oxidase and horseradish peroxidase for the assay of d-pipecolic acid. Electrode characteristics were obtained and compared for the different carbon paste biosensors at different working potentials. The linear concentration ranges for the proposed biosensors were in the fmol/l to μmol/l magnitude order with limits of detection of fmol/l to pmol/l magnitude order. The results obtained for enantioanalysis of l- and d-pipecolic acid in serum samples are correlating very good with those obtained using a standard method of analysis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
