Abstract

ABSTRACTAn amperometric ethanol sensor was constructed by co‐immobilization of alcohol dehydrogenase (ADH) and bovine serum albumin (BSA) in carbon paste containing entrapped NAD+. Conditions for optimum electrode response were determined by manipulation of experimental variables. Incorporation of both ADH and NAD+ in carbon paste resulted in better relationships between the enzymatic reaction and the electrochemical process. The surface of such an electrode could be renewed by polishing and smoothing on a clean paper. The sensor showed rapid response time and high sensitivity.

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