Biosensor Technology and the Clinical Biochemistry Laboratory – Issue of Signal Interference from the Biological Matrix

Abstract

This chapter discusses the potential use of biosensor technology in the clinical biochemistry laboratory. Various relevant key aspects of biosensor technology are introduced such as the chemistry of attachment of probes to device surfaces and a summary of the main categories of sensors based on electrochemistry, acoustic-wave physics and optical science. Important performance characteristics of typical clinical measurements are appraised with examples being presented. Following this discussion, the relevant issues of device selectivity, sensitivity, dynamic range and calibration with respect to target concentration, and possibility for label-free operation are evaluated. A critical issue for potential clinical measurement is the mandatory requirement for devices to function in biological fluids and matrices, with avoidance of signal interference caused by nonspecific surface adoption. Solutions for the latter problem are summarized. The chapter closes with a look at the possible features of biosensor technology that could be employed in the clinical biochemistry laboratory.