Abstract
Among various bioremediation methods, bioaugmentation is the one which frequently used. In this study, bioaugmentation was conducted by utilising Pseudomonas aeruginosa, Bacillus cereus, and Brevibacterium flavum. Many studies on and implementations of this method have been conducted. However, this method still needed to be studied especially with reference to its implementations to small automotive workshops, which their number was increasing, as the sources of the soil contamination. The objective of this study was to identify the most effective bacteria in removing total hydrocarbon content (TPH) from contaminated soils and to understanding several characteristics of the method. Laboratory research was set up and the experimental design was complete randomised design (CRD) with three replications. Statistical test of Anova with α 5% was carried out to evaluate the results of the study. The treatments of this study were three species of bacteria which were inoculated separately into the contaminated soils. The parameters were TPH, pH and the density of bacteria consortiums. The highest effective removal of TPH was showed by P. aeruginosa, 64,1% (δ+2,61%), followed by B. flavum and B cereus. This treatment had pH dropped two points and the density of bacteria consortium increased 6-10 times. The main limitation of this study was the number of parameters which was a few resulting difficulties in understanding the hydrocarbon degradation processes. The results of this study may give significant contributions to managers of small automotive workshops in cities which currently are not managed their spills properly.
Highlights
Keputusan Menteri (Kepmen) Lingkungan Hidup (LH) nomor 128 tahun 2003 tentang Tata Cara dan Persyaratan Teknis Pengelolaan Limbah Minyak Bumi dan Tanah Terkontaminasi oleh Minyak Bumi Secara Biologis, mensyaratkan bahwa limbah minyak bumi yang dihasilkan merupakan limbah Bahan Berbahaya dan Beracun (B3) yang berpotensi menimbulkan pencemaran atau kerusakan lingkungan, oleh karena itu perlu dilakukan pengelolaan dengan baik
bioaugmentation was conducted by utilising Pseudomonas aeruginosa
Statistical test of Anova with α 5% was carried out to evaluate the results of the study
Summary
Parameter tanah terkontaminasi di dalam penelitian ini yang dianalisis dan metode analisisnya disajikan seperti di dalam Tabel 2. Bakteri yang digunakan adalah Pseudomonas aeruginosa, Bacillus cereus, dan Brevibacterium flavum yang inokulumnya didapatkan dari Laboratorium Mikrobiologi dan Pangan Universitas Gadjah Mada, Yogyakarta. R1 = Reaktor berisi 300 g tanah terkontaminasi hidrokarbon + inokulum bakteri B. cereus 10% (v/w) 2. R2 = Reaktor berisi 300 g tanah terkontaminasi hidrokarbon + inokulum bakteri B. flavum 10% (v/w) 3. R3 = Reaktor berisi 300 g tanah terkontaminasi hidrokarbon + inokulum bakteri P. aeruginosa 10% (v/w) 4. Bahan utama penelitian ini adalah tanah terkontaminsi hidrokarbon dengan karakteristik seperti ditambilkan dalam Tabel 3
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