Abstract
Although research on the development of on-line HPLC systems has been conducted for many years, there is still no satisfactory solution available in the area of fully automatic sampling and sample preparation during fermentation processes. Sample preparation, which is often complicated, is of great importance for a successful application. A particular problem, for example, is still the reliable and robust dilution of the sample. The proposed system fulfills the requirement of fully automatic sampling and sample preparation by use of the biofiltrator and an autosampler with microrobotic-system. In addition to applications for on-line-HPLC, the same configuration can be applied to on-line FIA (Flow Injection Analysis). This double function leads to a new name: High-Pressure-Injection Analysis (HPIA). The selection of the individual components was largely based on convenient use and industrial suitability. Because of the modular structure of the system, it is possible to include other components at any time. Furthermore, it is even possible to use this application in other areas such as environmental analysis and process chromatography.
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