Bioprospecting of Red Algae (Rhodophyta) for bacterial infectious diseases in aquaculture

Abstract

Bacterial infectious diseases is one of major problem in aquaculture. Bioassay guided fractionation was employed to determine the antibacterial activity and to identify the chemical constituent of active fractions several red algae from Gunung Kidul, Indonesia. Bioassay was conducted against fish pathogen Vibrio spp., Aeromonas hydrophila, and Bacillus sp. using several methods which are diffusion agar, bioauthography, 6 and 96 well microplates. Profiling chemical constituent was done by thin layer chromatography and GC-MS. Result showed that Gracilaria edulis, Gracilaria arcuata Laurencia papilosa and Gellidium spinosum inhibited Vibrio sp, Aeromonas hydrophylla and Bacillus sp. Metabolites analysis using GC-MS yielded chemical constituents of active fractions such as phenolic compounds, terpenoid, 9-hexadecenoic acid, hexadecanoic acid, 13-octadecenoic acid, 10-octadecenoic acid, eicosanoic acid, 1,2-benzenedicarboxylic acid, cholest-8-en-3-ol and others. Our study concluded that red algae from Gunung Kidul, Yogyakarta, Indonesia is a potential sources of antibacterial for aquaculture application.