Bioprospecting of fruit extract and endophytic bacteria isolated from dewandaru (Eugenia uniflora L.) as antibacterial against colorectal bacteria

Abstract

Colorectal cancer (CRC) is the third most commonly diagnosed malignancy and the fourth leading cause of cancer-related deaths in the world. The gut bacteria are an important player in the development of colorectal cancer. Dewandaru (Eugenia uniflora L.) have been used as a traditional medicine to treat various diseases. Many of antibacterial metabolites produced by the fruit and its endophytic bacteria. This study aimed to find Antibacterial activity of ethanolic extract of dewandaru fruit using Kirby - Bauer disk diffusion method and to determine Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) using Dilution Tube Method (DTM). Endophytic bacteria were isolated from dewandaru fruit by using sterilization treatment followed by serial dilution agar plate and streak technique. All the isolates and ethanolic extract with different concentration were evaluated for the antimicrobial activity against colorectal bacteria (Streptococcus bovis, Enterococcus faecalis, Escherichia coli and Salmonella enterica). The results showed resistant– strong inhibitory zone category of fruit extract and isolated endophytic bacteria. The isolates were identified as Bacillus cereus, Bacillus amyloliquefaciens, and Bacillus sp.