Abstract
Introduction and Aim: The biosynthetic method of naturally occurring pigment melanin production has become a predominant technique in recent years owing to its cost-effectiveness, low chemical usage, and reduced purification procedure. This study aimed to investigate the significant production, characterization, and biological applications of fungal melanin pigment. Materials and Methods: Melanin production in Gliocephalotrichum sp. DSGB2 strain was carried out by submerged fermentation in tyrosine broth and further purified by acidification and precipitation methods. The purified melanin was characterized by analytical methods such as ultraviolet-visible absorbance, TLC, FTIR spectroscopy, and LC-MS. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging analysis was performed to evaluate antioxidant properties of melanin. Results: Pigment was confirmed as melanin based on tyrosinase enzyme assay, UV-visible spectroscopy absorbance, TLC, FTIR, and LC-MS analysis. The biosynthesis of melanin was optimized by varying the culture conditions, and the highest yield was obtained under pH 6 at 30ºC. The strain produced about 3.82 gL-1 of melanin in 5 days under optimum conditions and exhibited antioxidant activity. Conclusion: The study provides new ideas into the biosynthesis of water-soluble melanin by the fungal strain Gliocephalotrichum sp. DSGB2 has broad potential applications as an efficient biomaterial in the biopolymer, pharmaceutical sectors, cosmetic, and environmental.
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