Abstract
3D bioprinting is a potential technology for fabricating kidney and tracheal grafts since the matching donors is insufficient for many patients on demand. However, cell damage occurs due to variety factors during bioprinting process and is considered as a possible barrier for broad applications of delicate cells 3D bioprinting. Therefore, Cell viability is very challenging task in 3D bioprinting. The prime objective of current study is to explore effect of printing frequency on cell viability during printing process by a homemade Pneumatic Microdrop-on-Demand (PMOD) platform. Thus Human Embryonic Kidney 293 Cells (HEK293Cs) with diameter 15~20 μm and Human Bronchial Epithelial Cells (HBECs) with diameter 50~70 μm are printed to different sample tubes at frequencies of 10~70 Hz. Non-ejected cell suspension is categorized as control group. Then 7-AAD dye and flow cytometry method are utilized to evaluate the cell viability. It is found that the relative viabilities of HEK293Cs and HBECs are (0.990 ± 0.006) and (0.996 ± 0.014) respectively. Hence, it is concluded that the printing frequency has no significant effect on cell viability and PMOD cell printer reaches high cell viability due to the low shear stress during cell printing process along with no moving component in the bioink-filled chamber.
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