Biophototic in azuki bean seeds treated with ultrahigh dilutions

Jasper José Zanco,Pedro Boff,Simone Silmara Werner,Mari Ines Carissimi Boff

doi:10.33448/rsd-v10i2.12462

Abstract

Seed analysis is usually performed by destructive samples with time-consuming methods. Treatments that improve seed vigor are suggested based on bioassays and protocols for most plants. This includes the use of images to assess quality and pro- vide information that supports decisions. However, instrumental cost has not made these technologies widely used. The objective of this research was to study biophotonic images generated by low cost technologies - cold plasma scanner or gas discharge visualization (GDV) and speckle laser (BSL) - to evaluate the germination of adzuki beans seeds treated with ultrahigh dilutions (UHD). The research was carried out at the EPAGRI Homeopathy and Plant Health Laboratory, with a post-graduate support at the State University of Santa Catarina (UDESC). The images of the seeds were diagnosed mathematically, before and after germination, using “Local Connected Fractal Dimension Analysis” (LCFD) and “Time History Speckle Pattern” (THSP). The results show that the images identify the effects of high dilution - Bryonia alba and Silicea terra, at 6, 12 and 30 CH (centesimal hahnemannian dilution order) - on the seeds of azuki beans. There were statistically differences (p < 1% and p < 5%) of the treatments in relation to the control: water. Only Silicea terra 30CH inhibited seed germination. The signals found in the BSL images of the seeds were superior to the GDV signals, showing an average of 89% correct responses (BSL) against 87% (GDV).

Highlights

Plant propagules can be evaluated by conventional direct analysis methods, sample destruction, or by methods that conserve the physical integrity of these propagules by relating them to agronomic field attributes
The objective of the present research was to study the ultra-diluted and dynamized preparations effect of Silicea terra and Bryonia alba on adzuki-bean seeds by biophotonic analysis of images captured across low intensity laser (BSL) and gas discharge ionization of cold plasma (GDV), using free algorithms developed on the Java platform and available in ImageJ software plugins: “Local Connected Fractal Dimension Analysis (LCFD)” and “Time History Speckle Pattern (THSP)”
3.1 Pattern analysis on gas discharge visualization (GDV) and biospeckle laser (BSL) seed images The methods used for image analysis presented different responses according to the image capture method, GDV or BSL (Table 1), where the effects of ultrahigh dilution (UHD) on adzuki-bean seeds show biological activity through the captured images

Summary

Introduction

Plant propagules can be evaluated by conventional direct analysis methods, sample destruction, or by methods that conserve the physical integrity of these propagules by relating them to agronomic field attributes. Indirect seed analysis with traditional biophotonic instruments, such as the magnetic resonance imaging, Raman spectroscopy, UV, VIS, and NIR sensors make easy to predict the biological changes and they may preserve the samples (Bajpai, Bajpai & Roy, 1991; Thomas, 2015; Waisse, 2019). Conventional imaging tools which use destructive samples are generally expensive and ineffective in measuring the subtle effects of ultrahigh dilution (UHD) subjected to some biological signal (Bajpai et al, 1991; Matzrafi et al, 2017). The effects of UHD on plants change depending of the botanical species (phenotype and genotype), phytosanitary conditions and the biological and physical environment. Lettuce seeds submitted to toxic aluminum levels showed reversal of toxicity with application of Alumina 6CH and Calcarea carbonica 12CH (Bonfim, Casali, Mendonca & Martins, 2014)

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Results