Abstract

Introduction: Pesticide exposure causes acute and chronic adverse health effects on humans affecting the Kelch ECH-associated protein (Keap1)- nuclear factor erythroid 2-related factor 2 (Nrf2) and other oxidative pathways. We assessed the Keap1-Nrf2 pathway and other oxidative stress biomarker levels among a cohort of agricultural pesticide sprayers (SP) and nonspraying (NSP) farmers of a rural community during the cotton and wheat cropping seasons in India. Methodology: We randomly selected eight villages from the four blocks of Bathinda district, Punjab. Using a cohort study design, we collected the socio-demographic characteristics and biological samples (blood and urine) from 68 SP and 71 NSP at baseline, during the flowering seasons of cotton and wheat crops at scheduled time points. The United States Environment Protection Agency methods and standards were used to detect pesticide residues. Standard and validated enzyme-linked immunosorbent assay-based kits and colorimetric method-based kits were used to detect the biomarkers of Keap1-Nrf2 and oxidative stress pathways. Results: In Keap1-Nrf2 pathway, cotton season glutathione peroxidase (P = 0.004) and baseline heme oxygenase-1 (P Conclusion: The Keap1-Nrf2 and other oxidative stress biomarker levels between the group were not consistently different all times across the seasons. The biomarker levels of SP and NSP need to be compared with the nonagricultural population.

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