Biomedical Applications of Colloidal Nanocrystals

Marek Osinski,Thomas M Jovin,K Yamamoto

doi:10.1155/2007/82752

Abstract

Biomedical Applications of Colloidal Nanocrystals

Highlights

For the study on the ligand-receptor binding, we need fluorescent markers
Homogenized buffer solutions of QDs capped with mixture of DHLAPEG600 and dihydrolipoic acid (DHLA)-tetraethylene glycol (TEG)-biotin are stable over broad pH range and showed specific interactions with NeutrAvidin in surface binding assays
Tomlinson et al for imaging GABAc receptor on the surface membrane of intact cell. They have already succeeded in developing serotonin-conjugated quantum dots and the method to reduce nonspecific binding using PEGylated quantum dots. Together with all these works, they have proceeded for imaging GABAc receptors heterologously expressed on Xenopus laevis oocytes

Summary

Introduction

For the study on the ligand-receptor binding, we need fluorescent markers. Ligand conjugated quantum dots are reported here by Ian D. The investigation contains the following three results: firstly, the precise detection of relative changes in size upon binding of molecules, secondary, comparison of the specific attachment of DNA via gold-thiol bonds to Au nanoparticles with nonspecific adsorption of DNA, and thirdly, determination of the maximum number of DNA molecules bounded per particle. Homogenized buffer solutions of QDs capped with mixture of DHLAPEG600 and DHLA-TEG-biotin are stable over broad pH range and showed specific interactions with NeutrAvidin in surface binding assays.

Results

Conclusion