Abstract
Objective To investigate the histocompatibility of the type Ⅰ collagen (CI)/vascular endothelial growth factor (VEGF) slow-release material.Methods Bone marrow-derived mesenchymal stem cells (BMSCs) were obtained with passage isolation,and then seeded on cross-linked CI,CI + VEGF materials and cell culture plate.Cytocompatibility was detected by using scanning electron microscopy (SEM) at day 5 and cell counting kit-8 (CCK-8) test at every day during 10 days after seeding.The CI and CI + VEGF materials were subcutaneously implanted into 8 New Zealand albino rabbits.The rabbits were sacrificed at 7,14,21 and 28 days and the scaffolds and the surrounding tissues were extracted for hematoxylin-eosin (HE) staining.Results CCK-8 test revealed that the cell proliferation in 3 groups was as follows:CI + VEGF group ＞ CI group ＞ common culture group,and the cell viability in CI + VEGF group was significantly higher than that in controls (day 4:2.77 ±0.21 ＞2.21 ±0.17 ＞ 1.86 ±0.13 P ＜ 0.05 ; day 8:2.47 ± 0.19 ＞ 1.89 ± 0.15 ＞ 1.16 ± 0.11,P ＜ 0.05).SEM image of the scaffolds showed that BMSCs spread well and homogenously distributed throughout the entire scaffolds.Histological HE staining showed that the immune rejection of CI + VEGF sponge material was similar to CI,and CI + VEGF sponge material could be degradated at 28 days in animals.Conclusion CI + VEGF sponge materials have well histocompatibility. Key words: Type Ⅰ collagen; Bone marrow-derived mesenchymal stem cells; Vascular endothelial growth factor; Histocompatibility
Published Version
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