Biomechanical properties of the layered oesophagus and its remodelling in experimental type-1 diabetes

Abstract

Passive biomechanical properties in term of the stress–strain relationship and the shear modulus were studied in separated muscle layer and mucosa–submucosa layer in the oesophagus of normal and STZ (streptozotocin)-induced diabetic rats. The mucosa–submucosa and muscle layers were separated using microsurgery and studied in vitro using a self-developed test machine. Stepwise elongation and inflation plus continuous twist were applied to the samples. A constitutive equation based on a strain energy function was used for the stress–strain analysis. Five material constants were obtained for both layers. The mucosa–submucosa layer was significantly stiffer than the muscle layer in longitudinal, circumferential and circumferential–longitudinal shear direction. The mechanical constants of the oesophagus show that the oesophageal wall was anisotropic, the stiffness in the longitudinal direction was higher than in the circumferential direction in the intact oesophagus ( P < 0.001 ) and in the muscle layer ( P < 0.05 ). Diabetes-induced pronounced increase in the outer perimeter, inner perimeter and lumen area in both the muscle and mucosa–submucosa layer. The growth of the mucosa–submucosa layer ( P < 0.001 ) was more pronounced than the muscle layer ( P < 0.05 ). Furthermore, the circumferential stiffness of the mucosa–submucosa layer increased 28 days after STZ treatment. In conclusion, the oesophagus is a non-homogeneous anisotropic tube. Thus, the mechanical properties differed between layers as well as in different directions. Morphological and biomechanical remodelling is prominent in the diabetic oesophagus.