Biomarkers of oxidative stress and antioxidant defense system among Guatemalan preschool children attending daycare centers with a common menu offering (639.2)

Abstract

Objective: To describe and compare urine oxidative stress biomarkers and erythrocyte antioxidant enzymes activity, in preschoolers from 3 daycare centers in Guatemala Methods: 74 (2‐6 y/o) children (36 F / 38 M) attending 3 government‐subsidized daycare centers identified by location, coded as Center (C) and as to unique location: A (semi‐urban n=19); B (marginal‐urban n=23); and C (rural n=32) enrolled to measure oxidative damage to DNA (8‐hydroxydeoxyguanosine {8‐OHdG}) and lipid (15‐Isoprostane F2t {F2‐Iso}) and the erythrocyte activity of Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione Reductase (GSHR) and Peroxidase (GPx) Results: 8‐OHdG and F2‐Iso median values were different between sexes (p=0.03 and p=0.04, respectively), even when calculating the total amount excreted per day and adjusting to weight. GPx also differed by sex (p=0.01). According to location 8‐OHdG and F2‐Iso were different between CA and CB (p<0.01 and p<0.01, respectively). CAT and GSHR were different in CB when compared to CA and CC (p<0.01 in both cases). SOD activity was different among all 3 centers (p<0.01), whereas GPx was different between CB and CC (p=0.02) Conclusion: Differences in oxidative biomarkers between sexes, especially those biomarkers that are involved in lipid oxidation (F2‐Iso and GPx), are seen.Funded by: Fundación Iberoamericana de Nutrición (FINUT), Spain and The Hildegard Grunow Foundation (HGF), GermanyGrant Funding Source: Supported by Fundación Iberoamericana de Nutrición (FINUT) and The Hildegard Grunow Foundation (HGF)