Abstract
Objective of the study was to investigate biological properties and genetic characteristics of tularemia agent strains isolated from natural foci of the Rostov Region in 2020.Materials and methods. Field material from natural foci of the Rostov Region was examined by serological, bacteriological, biological, and molecular-genetic methods. Cultural-morphological, biochemical, antigenic and pathogenic properties of isolated cultures were studied. Protein profles were obtained through MALDI-TOF MS using mass spectrometer Autoflex speed III Bruker Daltonics and Flex Control of Biotyper software. The genetic characteristics of the strains were determined by VNTR and INDEL typing and SNP analysis.Results and discussion. Six strains of tularemia pathogen were isolated from mouse-like rodents using biological method. The investigation of their biological features and data of PCR analysis and INDEL typing with canonical markers showed that all strains are typical representatives of the Francisella tularensis subsp. holarctica biovar EryR. VNTR typing by six genetic loci revealed that all strains belong to four individual genotypes. The strain isolated in 2020 in the Salsky district was identical to the strain which was isolated in the same area in 1989. Based on the whole genome sequencing of two strains, we established that they are closest to the cultures isolated in Turkey (2009, 2012) and Khanty-Mansiysk (2013) by the studied set of SNP markers. Thus, we found that both identical (or closely related) clones of the tularemia agent and new strains with unique genotypes which previously were not described for the Rostov Region can circulate in natural foci of this region for a long period of time.
Highlights
We found that both identical (or closely related) clones of the tularemia agent and new strains with unique genotypes which previously were not described for the Rostov Region can circulate in natural foci of this region for a long period of time
Gyuranecz M., Birdsell D.N., Splettstoesser W., Seibold E., Beckstrom-Sternberg S.M., Makrai L., Fodor L., Fabbi M., Vicari N., Johansson A., Busch J.D., Vogler A.J., Keim P., Wagner D.M. Phylog eography of Francisella tularensis subsp
Summary
БИОЛОГИЧЕСКИЕ СВОЙСТВА И ГЕНЕТИЧЕСКАЯ ХАРАКТЕРИСТИКА ШТАММОВ FRANCISELLA TULARENSIS, ИЗОЛИРОВАННЫХ НА ТЕРРИТОРИИ РОСТОВСКОЙ ОБЛАСТИ В 2020 г. Цель работы – изучение биологических свойств и генетических характеристик штаммов возбудителя туляремии, изолированных в природных очагах Ростовской области в 2020 г. Генетические характеристики штаммов определяли методами VNTR- и INDEL-типирования и SNP-анализом. При мониторинге природных очагов туляремии от мышевидных грызунов с помощью биологической пробы выделены шесть штаммов возбудителя туляремии. Что по своим биологическим свойствам, а также в соответствии с данными ПЦР-анализа и INDEL-типирования по каноническим маркерам все штаммы являются типичными представителями вида Francisella tularensis subsp. Установлено, что в природных очагах Ростовской области в течение длительного времени могут циркулировать как идентичные (или близкородственные) клоны возбудителя туляремии, так и появляться новые штаммы с уникальными генотипами, ранее не описанными для Ростовской области. Ключевые слова: Francisella tularensis, выделение штаммов, биологические свойства, INDEL-типирование, VNTR-типирование, SNP-типирование.
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