Biological evaluation of recombinant human bone morphogenetic protein-2 artificial bone in lumbar intertransverse process fusion

Abstract

Objective To evaluate rhBMP-2/20%β-TCP/PDLLA complex biological properties.Methods Preparation of casting salting was carried out using three-dimensional porous 20% rhBMP-2/β-TCP/PDLLA. 24 4-month-old healthy male New Zealand rabbits were randomly divided into A,B groups of 12,according to the method described in Boden production L4,5 intertransverse process fusion model. A group was embedding rhBMP-2/20%β-TCP/PDLLA complex,and B group was implanted autogenous iliac bone block. Animals were killed according to postoperative time(3 weeks,6 weeks, 12 weeks) ,for gross observation, X-ray observation of the formation of new bone, artificial bone degradation, and early histological manifestations. Results Composite artificial bone and autologous iliac bone graft were able to achieve good spinal fusion,artificial bone graft where there is no inflammatory response,but also in new bone formation,while reshaping the gradual degradation of carrier material. Conclusions 20%β-TCP/PDLLA showed good biocompatibility. Its structure and micro-environment created by its degradation were conducive to play a role for the rhBMP-2. β-TCP/PDLLA had strong activity in inducing bone and good bone fusion ability,and it was a promising bone graft substitutes, which fusion power was stronger than that in autologous iliac bone graft. Key words: Bone morphogenetic porteins; Bone substitutes; Spinal fractures; Biomechanics