Abstract
Background: The aim of this study was: to evaluate the biological properties of new hydraulic materials: Bio-C Repair and Bio-C Sealer. Methods: Periodontal ligament stem cells were exposed to several dilutions of Bio-C Repair and Bio-C Sealer. The ion release profile and pH were determined. Metabolic activity, cell migration and cell survival were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing assays and Annexin assays, respectively. Cells were cultured in direct contact with the surface of each material. These were then analyzed via scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Statistical differences were assessed using a two-way ANOVA (α < 0.05). Results: Similar pH was observed in these cements. Bio-C Sealer released significantly more Ca and Si ions (p < 0.05) in comparison with Bio-C Repair. Undiluted Bio-C Sealer induced a significant reduction on cellular viability, cell survival and cell migration when compared to the control (p < 0.05). Moreover, SEM showed abundant cells adhered on Bio-C Repair and a moderate number of cells attached on Bio-C Sealer. Finally, EDX analysis identified higher percentages of Ca and O in the case of Bio-C repair than with Bio-C sealer, while other elements such as Zr and Si were more abundant in Bio-C sealer. Conclusions: Bio-C Repair displayed higher cell viability, cell adhesion and migration rates than Bio-C Sealer.
Highlights
Hydraulic materials, known as hydraulic silicate-based materials or bioactive cements (BECs), are new materials which leach ions, with potential influence on reparative/regenerative responses [1]
Known as hydraulic silicate-based materials or bioactive cements (BECs), are new materials which leach ions, with potential influence on reparative/regenerative responses [1]. These new materials represent an improvement over mineral trioxide aggregate MTA-based materials by minimizing drawbacks such as tooth discoloration, relatively difficult handling, long setting times or the release of heavy metal elements [2,3,4]
BECs tested in this study were Bio-C Repair and Bio-C Sealer (Angelus, Londrina, Brazil)
Summary
Known as hydraulic silicate-based materials or bioactive cements (BECs), are new materials which leach ions, with potential influence on reparative/regenerative responses [1]. Two new materials Bio-C Repair (repair cement) and Bio-C Sealer (endodontic sealer) (Angelus, Londrina, PR, Brazil) were placed on the market in the form of premixed bioceramic materials with the same biological interactions as mineral trioxide aggregate but providing improvements in terms of manipulation and insertion. These materials contain calcium silicates, which are hydrated by contact with the local humidity, producing a hydrated calcium silicate structure and calcium and hydroxyl ions, incorporating zirconium oxide as a radiopacifier [8]. Conclusions: Bio-C Repair displayed higher cell viability, cell adhesion and migration rates than Bio-C Sealer
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