Abstract
Background: Cancer is constituted as the most famous fatal disease among human-being. DMBA is a common carcinogen and immunosuppressant and experimentally used for the induction of mutations via the creation of DNA adducts. Aim: Evaluation of the therapeutic effect of Lagenaria siceraria on 9,10 dimethyl-1, 2-benzanthracene (DMBA)-induced changes. Materials and Methods: A total number of thirty adult female albino rats within average age 3 months classified into 3 groups as follow: Group1 is designated as control. Group 2 was subcutaneously injected with a single dose of DMBA (10 mg/ 100 g b. wt) and after 90 days, animals have received a daily injection of distilled water for 30 days. Group 3 was subcutaneously mice were injected with a single dose of DMBA and after 90 days, mice were received morning and night daily injection of Lagenaria siceraria (100 mg/ 100 g b.w) for 30 days. After scarifying the mice, ileum samples were taken and passed the histological examination, also Histochemical stains were done. Results: From the cell biological, histochemical, and histopathological points of view, the carcinogen DMBA has a highly stimulatory effect on the cellular activities, DNA synthesis, RNA synthesis, and cell proliferative in the normal epithelial cells lining villi of the ileum of female mice, while Lagenaria siceraria has a highly inhibitory effect on the volume of the nuclei, DNA synthesist, and the cell proliferation of the DMBA-induced cells lining villi of the ileum of female mice. Conclusion: Finally, it could be said that the carcinogen DMBA has a proliferative and/or possibly a carcinogenic effect on the ileum of female mice. Also, It could be concluded that Lagenaria siceraria could be offered an antiproliferative and/or possibly an anti-carcinogenic effect against DMBA.
Highlights
Malignancy is one of the most frequent causes of mortalities world widely recorded in humans
The present data showed that the carcinogen DMBA highly increased the volume of the nuclei, DNA content, RNA content, and cell proliferation in the normal epithelial cells lining villi of the ileum of the female mice
While Lagenaria siceraria highly decreased the volume of the nuclei, DNA content, and cell proliferation and changed the contents of RNA, protein, and collagen in the epithelial cells lining villi of the ileum of the DMBA-induced female mice
Summary
Malignancy is one of the most frequent causes of mortalities world widely recorded in humans. Malignant neoplasm is a disease; since a crowd of cells has undergone abnormal growth sometimes termed metastasis (Samudral et al, 2015). This disease once attacks the body; it begins to grow without control (Jones and Johnson, 2012). Carcinogenesis is defined as multiple processes of developing cancer due to exposure to carcinogenic substances. It passed many stages including initiation, promotion, and progression. Group 3 was subcutaneously mice were injected with a single dose of DMBA and after 90 days, mice were received morning and night daily injection of Lagenaria siceraria (100 g/ 100 g b.w) for 30 days. It could be concluded that Lagenaria siceraria could be offered an antiproliferative and/or possibly an anticarcinogenic effect against DMBA
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