Abstract
To test the antagonistic ability of <i>Bacillus amyloliquefaciens</i> HY against lettuce sclerotinia rot caused by <i>Sclerotinia sclerotiorum</i>, an <i>in vitro</i> test, greenhouse test and growth chamber test were conducted. As a result of <i>in vitro</i> dual culture test for sclerotia of <i>B. amyloliquefaciens</i> HY, the mycelial growth inhibition rate was 70.7%. Mycelial tip was swollen, black, and had an irregular and deformed shape. In I-plate test, the antagonistic volatile compounds produced by <i>B. amyloliquefaciens</i> HY, the mycelial growth inhibition rate was 57.7% and the sclerotia germination inhibition rate was 46.7%. In addition, <i>B. amyloliquefaciens</i> HY had sideropore production capacity, phosphate solubillibty, and protease production capability, but <i>B. amyloliquefaciens</i> HY did not produce chitinase. In growth chamber test, the lettuce survival rate was 0% in the control group treated with only the pathogen, and 80% in the treatment group treated with of <i>S. sclerotiorum</i> and <i>B. amyloliquefaciens</i> HY by diluting 10 times of the lettuce survival rate. In addition, the lettuce survival rate of 80% was shown in the treatment group treated with the culture medium of <i>B. amyloliquefaciens</i> HY 50 times 1 week later after inoculation with <i>S. sclerotiorum</i>.
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