Biological Control of Aedes aegypti Larvae using Sea Shell Powder

Abstract

Background: The Aedes aegypti mosquito is a primary vector for several arboviral diseases, including dengue, Zika, chikungunya and yellow fever, posing significant public health challenges, especially in tropical and subtropical regions. Traditional chemical insecticides have been the mainstay for controlling Aedes aegypti populations, but their extensive use has led to the development of insecticide resistance, environmental pollution and adverse effects on non-target organisms. This study explores the potential of sea shell powder as a natural larvicide against Aedes aegypti larvae. Methods: Sea shell powder, derived from the calcified exoskeletons of marine mollusks, was tested for its larvicidal activity against fourth instar larvae of Ae. aegypti at concentrations ranging from 100 to 600 ppm. Result: The results demonstrated a concentration and time-dependent increase in larval mortality, with LC50 values of 407.01 ppm at 24 hours and 327.55 ppm at 48 hours. The findings suggest that sea shell powder could serve as an effective, environmentally sustainable alternative to chemical insecticides, offering advantages such as biodegradability, low cost and reduced risk of resistance development. This study supports the potential inclusion of sea shell powder in integrated mosquito management programs, particularly in areas with prevalent insecticide resistance.