Abstract
This study was performed to investigate the biocompatibility (BC), magnetothermal effect, and DNA binding biological characteristics of manganese zinc ferrite nanoparticles (Mn0.6Zn0.4Fe2O4-NPs (MZF-NPs)) coated with pegylated manganese (PEG-MZF-NPs). Their functions as gene transfer carrier for gene therapy and magnetic medium for tumor hyperthermia were also explored. The manganese zinc ferrite nanoparticles were synthesized through high temperature cracking, and their characterizations were discovered. Hemolysis test and MTT assay were performed to evaluate biocompatibility, and their self-heating effects in the alternating magnetic field were investigated. PEG-MZF-NPs with different concentrations were measured by using 7.0 T Micro-MR scanner (MRI) to calculate the T2 value and r2 relaxation rate of each sample. The CD44-shRNA plasmids were constructed, and their ability to bind PEG-MZF-NPs were examined. The DNA release from PEG-MZF-NP/DNA complex and protection of DNA from nuclease digestion were also detected. After CD44-shRNA-EGFP were transfected into the ovarian cancer SK-OV-3 cells by using PEG-MZF-NPs as carriers, the transfection efficiency was detected by a flow cytometer and expression of CD44 mRNA and protein in cells was detected using RT-PCR and Western blot, respectively. We successfully prepared PEG-MZF-NPs with favorable dispersity, magnetic responsiveness, and BC. Typically, the excellent magnetothermal effect can be used for a tumor magnetothermal therapeutic study. In vitro MRI showed the application potential for being magnetic resonance T2 relaxation contrast agents and the possibility to achieve goal of integration of targeting diagnosis and treatment. The CD44-shRNA plasmids have been successfully constructed and concluded that PEG-MZF-NPs may serve as gene transfer carriers for gene therapy.
Highlights
With the development of nanotechnology, nanoparticles (NPs) used as gene and drug carrier have been extensively studied [1, 2]
In the present study, when manganese zinc ferrite nanoparticles were modified with polyethylene glycol (PEG), they had favorable water solubility and biocompatibility, which could be used as a gene or drug carrier
T2 values of PEG-Mn0.6Zn0.4Fe2O4 nanoparticles (MZF-NPs) at different concentrations were measured, and the relaxation rate r2 of materials was 6.06 μg·mL-1 s-1 (Figure 4(b)). These findings indicate a good potential for the MRI contrast agent
Summary
With the development of nanotechnology, nanoparticles (NPs) used as gene and drug carrier have been extensively studied [1, 2]. Apart from the general functions of a nanocarrier, magnetic nanoparticles can produce a thermal effect in an alternating magnetic field and may be used for tumor hyperthermia [4, 5]. Magnetic iron oxide (Fe3O4, Fe2O3) nanoparticles are widely used metallic oxides. Manganese zinc ferrite (Mn0.6Zn0.4Fe2O4, Mn0.5Zn0.5Fe2O4) nanoparticles have been used as vectors in suicide gene HSV-TK therapy for hepatoma and showed a satisfactory effect [6]. It is considered that manganese zinc ferrite nanoparticles may be used as a vehicle for CD44shRNA therapy and function as a magnetic medium for magnetic thermotherapy on ovarian cancer
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