Biological Applications of Nano Cellulose-ZnO-Ag Composite and its Ingredients as Antioxidant, Anticoagulant and Inhibitor of Cell Proliferation of Breast Cancer

Abstract

Bacterial cellulose (BC) is cellulose that is formed by microbial strains. The objectives of this research are to determine the antioxidant, anticoagulant, and cytotoxicity profile of a microbial Nano cellulose-ZnO-Ag composite and its components separately. Silver nanoparticles (AgNPs) (IC50 65 ug/ml) were found to have the highest antioxidant activity, followed by CNCs-ZnO-Ag composite (IC50 88.98 ug/ml), Oxide zinc nanoparticles (ZnONPs) (IC50 263 ug/ml), and BC (IC50 955 ug/ml). The PT test increase as the concentrations of the samples increase eventually reaching 75 µgml-1 for all samples. Furthermore, the CNCs-ZnO-Ag composite, BC, and AgNPs at 25 µg ml-1 had clotting times that were nearly identical to the control. The APTT test at 75 μg/ml of CNCs-ZnO-Ag composite plasma clotting time increased (56 Sec) in relation to the control one which recorded 33 Sec. The in vitro cytotoxicity of CNCs- ZnO-Ag composite and its components separately were performed by MTT assay against MCF-7 breast cancer cells at various concentrations for each compound. The lowest IC 50 was observed of CNCs-ZnO-Ag at 69.12 μg ml−1 and the highest at 396.81 μg ml−1 of BC. On the other hand, at the same concentration of CNCs-ZnO-Ag composite no activity was found against normal fibroblast Wi-38 cell.