Biological activity of Pomegranate peels Punica granatum Silver nanoparticles AgNPs extract against Fourth larvae of Culex quinquefasciatus mosquito (Diptera: Culicidae)

Abstract

Present study was included the assessment of biological activity of Pomegranate Peels Punica granaatum plant extract toward fourth Culex quinquefasciatus larvae mosquito (Order: Diptera, Family: Culicidae) by testing ethanolic and Silver nanoparticles extracts. Results of present study revealed that Silver nanoparticles extract was more toxic than ethanolic extract and the mortality rates were reached to 90 % after 72 h exposure time when it treated with 5 µg/ml compared to 0% at control treatments. Ethanolic extract also was toxic at concentration 5 µg/ml and mortality rates were reached to 80% after 72 h exposure time. Study of larvae after death showed that penetration of Silver nanoparticles extract was entered inside mid gut of larvae after damage of body wall. SEM analysis indicated to that more deformation found in body wall thus AgNPs were easily penetration through skin of fourth larvae. SEM analysis also was showed that Silver nanoparticles were clustered and irregular or oval shapes.