Biological Activities of Thymine extracted from Ethyl Acetate of Cordyceps neovolkiana DL0004 isolated in Vietnam

Abstract

Cordyceps neovolkiana DL0004, one of the members of Cordyceps genus is widely found in the Southern region of Vietnam, the results showed that both ethyl acetate extract and thymine exhibited an anti-inflammatory effect via inhibition of NO production (IC50= 24.00 ± 1.07 and 19.66 ± 0.69 μg/ml, respectively) as well as regulation of NF-kB pathway through p50 and p65 expression. Furthermore, the extract and thymine also prevented DNA damage (>80%) from oxidative stress at the concentration of 50 μg/ml and 10 μg/ml respectively. Thymine and the extract could inhibit oxidation of membrane protein with IC50 = 4.89 and IC50 = 8.119 ppm respectively. These findings suggest thymine as potential candidate for the treatment of inflammation and oxidative stress-associated disorders.