Bioinformatics analysis of TYR within and among species

Abstract

The comprehensive bioinformatics of TYR within and among species was analyzed based on variation of nucleotide and amino acid sequence. The stop codon, TAA was used in Sus scrofa, Pan troglodytes, Canis familiaris, Equus caballus, Felis catus,Macaca mulatta, Oryctolagus cuniculus, and Homo sapiens, but thermal gravimetric analysis (TGA) in Bos taurus, Ovis aries, and Mus musculus. The phylogenetic analysis showed that there was the closest relationship between Homo sapiens and P. troglodytes, and the farmost between M. muculus and O. aries. The signal peptide and the transmembrane domain of TYR gene for species were found at 1 to 20 AA and 470 to 500 AA, respectively. The five continuous random coils (5c) in TYR secondary structure ofO. aries were found due to the substitution of polar Thr (T) for nonpolar Ile (I) and Leu (L) compared with other species. There was also a secondary structure variation of 2 c in H. sapiens due to the Pro that could break off the alpha helix simultaneous with a kink in polypeptide chain. The TYR is a kind of hydrophilic protein based on the hydropathy profile analysis, and the N-terminal and C-terminal hydrophobic region represents the signal peptide and the transmembrane domain, respectively. Key words: TYR gene, bioinformatics and species.