Abstract
LIM and SH3 domain protein (LASP-1) is responsible for the development of several types of human cancers via the interaction with other proteins; however, the precise biological functions of proteins interacting with LASP-1 are not fully clarified. Although the role of LASP-1 in hepatocarcinogenesis has been reported, the implication of LASP-1 interactors in HBV-related hepatocellular carcinoma (HCC) is not clearly evaluated. We obtained information regarding LASP-1 interactors from public databases and published studies. Via bioinformatics analysis, we found that LASP-1 interactors were related to distinct molecular functions and associated with various biological processes. Through an integrated network analysis of the interaction and pathways of LASP-1 interactors, cross-talk between different proteins and associated pathways was found. In addition, LASP-1 and several its interactors are significantly altered in HBV-related HCC through microarray analysis and could form a complex co-expression network. In the disease, LASP-1 and its interactors were further predicted to be regulated by a complex interaction network composed of different transcription factors. Besides, numerous LASP-1 interactors were associated with various clinical factors and related to the survival and recurrence of HBV-related HCC. Taken together, these results could help enrich our understanding of LASP-1 interactors and their relationships with HBV-related HCC.
Highlights
LIM and SH3 domain protein (LASP-1) is a scaffold protein that has been identified to facilitate the development of several types of human cancers[1], including breast carcinoma[2], prostate carcinoma[3], colorectal carcinoma[4], gastric cancer[5], oesophageal squamous cell carcinoma[6], and gallbladder cancer[7]
The gene expression and potential regulatory factors of LASP-1 interactors were further investigated using a microarray of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) tissues that was downloaded from the NCBI gene expression omnibus (GEO) database to identify candidate genes that may contribute to the development of HBV-related HCC in conjunction with LASP-1
LASP-1, the information of target human proteins was retrieved from different public databases, including IntAct[19], BioGRID20, APID21, PINA2.022, Mentha[23], HitPredict[24], WiKi-Pi25, PIPs26, PPI-finder[27] and PrePPI28 or from the studies reported in PubMed
Summary
LIM and SH3 domain protein (LASP-1) is a scaffold protein that has been identified to facilitate the development of several types of human cancers[1], including breast carcinoma[2], prostate carcinoma[3], colorectal carcinoma[4], gastric cancer[5], oesophageal squamous cell carcinoma[6], and gallbladder cancer[7]. LASP-1 contains an N-terminal LIM domain, two nebulin-like repeats named R1 and R2 domain, and a C-terminal SH3 domain[1,11] These unique domains facilitate its interaction with a variety of proteins. A study from the same group showed that LASP-1 interacts with S100 calcium binding protein A11 (S100A11) and increases its expression in CRC cells. The interaction of LASP-1 with S100A11 is required for EMT as well as progression of CRC13. Taken together, these results indicate that elucidating the www.nature.com/scientificreports/. The results from Wang H et al indicate that the increase of LASP-1 in HCC tissues is related to hepatitis B virus (HBV) infection[14]. The gene expression and potential regulatory factors of LASP-1 interactors were further investigated using a microarray of HBV-related HCC tissues that was downloaded from the NCBI gene expression omnibus (GEO) database to identify candidate genes that may contribute to the development of HBV-related HCC in conjunction with LASP-1
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