Abstract
Endophytic microorganisms absorb nutrients and prevent pathogen damage, supporting healthy plant growth. However, the relationship between endophytic bacteria and berberine synthesis in the medicinal plant Coptis teeta Wall. remains unclear. Herein, we explored the community composition of endophytic bacteria related to berberine in roots, stems, and leaves of wild-type and cultivated C. teeta. Endophytic bacterial communities were analyzed by 16S rRNA sequencing, and berberine content in roots was analyzed by high-performance liquid chromatography. Proteobacteria, Actinobacteria, and Bacteroidetes were the major phyla, and Mycobacterium, Salmonella, Nocardioides, Burkholderia-Paraburkholderia, and Rhizobium were the dominant genera in root, stem, and leaf tissues. Root berberine content was positively correlated with total N, total P, total K, and available K in rhizosphere soil. In addition, root berberine content was positively correlated with Microbacterium and norank_f_7B-8, whereas soil total K was positively correlated with Microbacterium and Burkholderia-Paraburkholderia in roots. Our results demonstrated a clear correlation between dominant endophytic bacteria and berberine synthesis in C. teeta. The findings are useful for the promotion of berberine production in C. teeta via manipulation of endophytic bacteria.
Highlights
Coptis teeta Wall. belongs to the genus Ranunculaceae and is considered an important medicinal plant in the Gaoligongshan area of northwestern Yunnan
The results revealed that the root endophytic Chao index of Coptis teeta Wall. was largest (p
We investigated the composition of bacteria in individual samples, and compared endophytic bacteria in the roots, stems and leaves of WT and cultivated plants using analysis of variance (ANOVA)
Summary
Coptis teeta Wall. belongs to the genus Ranunculaceae and is considered an important medicinal plant in the Gaoligongshan area of northwestern Yunnan. Belongs to the genus Ranunculaceae and is considered an important medicinal plant in the Gaoligongshan area of northwestern Yunnan. In. Chinese herbal medicine, Coptidis rhizome refers to the dried rhizome of Coptis chinensis Franch., C. deltoidea CYCheng et Hsiao, and C. teeta Wall. As long ago as the Ming Dynasty, Lan Mao recorded in the Diannan Materia Medica that Coptidis rhizome grown in Yunnan, namely Coptis teeta. Is widely considered to produce the best quality Coptidis rhizome. In addition to use in Chinese medicine, Coptidis rhizome is a source of compounds used in proprietary Chinese pharmaceutical products such as Huanglian 9 and Yinglian tablet. There are numerous pharmaceutical preparations containing Coptidis rhizome as a raw ingredient
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