Abstract
BackgroundOsteoarthritis (OA) is the most common arthritic disease in humans, affecting the majority of individuals over 65 years of age. The aim of this study is to identify the gene expression profile specific to subchondral bone in OA by comparing the different expression profiles in experimental and sham-operation groups.MethodsGene expression profile GSE30322 was downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were obtained by limma package. And Database for Annotation, Visualization and Integrated Discovery (DAVID) databases were further used to identify the potential gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, a protein–protein interaction (PPI) network was constructed and significant modules were extracted.ResultsTotally, 588 DEGs were identified including 199 upregulated DEGs and 389 downregulated DEGs screened in OA and sham-operation. GO showed that DEGs were significantly enhanced for ribosomal subunit export from nucleus and molting cycle. KEGG pathway analysis revealed that target genes were enriched in thiamine metabolism.ConclusionThese key candidate DEGs that affect the progression of OA, and these genes might serve as potential therapeutic targets for OA.
Highlights
Osteoarthritis (OA) is a degenerative disease characterized by the gradual degeneration of articular cartilage, joint stiffness, and loss of function [1]
588 Differentially expressed genes (DEGs) were identified including 199 upregulated DEGs and 389 downregulated DEGs screened in OA and sham-operation
Top 10 up-DEGs and down-DEGs were listed in Table 1 and Table 2, respectively
Summary
Osteoarthritis (OA) is a degenerative disease characterized by the gradual degeneration of articular cartilage, joint stiffness, and loss of function [1]. Subchondral bone alteration present to the cartilage degeneration and more. It has been stated that most of the OA patients accompanied by the alterations of the subchondral bone [4]. Subchondral bone could transport nutrients or cytokines to the overlying cartilage. Subchondral bone cells contacted with chondrocyte and influence cartilage metabolism. A better understanding of the early molecular mechanism changes of subchondral bone in vivo may contribute to elucidating the pathogenesis of OA. Osteoarthritis (OA) is the most common arthritic disease in humans, affecting the majority of individuals over 65 years of age. The aim of this study is to identify the gene expression profile specific to subchondral bone in OA by comparing the different expression profiles in experimental and sham-operation groups
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
